The crash occurred near Sanabria in the Zamora region, at the 65th kilometer of the A-52 highway.
Tragically, Jota had married Rute Cardoso just 10 days ago, sharing wedding photos that deeply saddened his fans.
Jota was traveling with his 26-year-old brother André when their car veered off the road and caught fire. Witnesses reported the vehicle was engulfed in flames.
His 25-year-old brother André Silva played for regional league teams in Portugal after progressing through Porto and Boavista youth systems.
He was loaned to Porto and Wolverhampton before making a breakthrough in the Premier League, leading to his 2020 transfer to Liverpool for €44.7 million.