United Nations Secretary-General Antonio Guterres on Tuesday condemned Israel's strikes as a "flagrant violation of the sovereignty and territorial integrity of Qatar."
He said Qatar has been playing a very positive role to try and achieve a ceasefire in Gaza and the release of all hostages held by Hamas.
"All parties must work towards achieving a permanent ceasefire, not destroying it," Guterres told reporters.
On Tuesday, Israel launched an airstrike on senior Hamas leaders in the Qatari capital Doha, a move seen as a fresh blow to efforts for a Gaza ceasefire deal.
Explosions were heard in Doha, with plumes of smoke seen rising into the sky.