Stephane Sejourne, who leads President Emmanuel Macron's centrist party, said de Fournas should resign, and the left-wing Nupes alliance said that he should be expelled.
Over the past years, Le Pen has made huge progress in detoxifying her party's image and convincing voters that the party founded by her father Jean-Marie, who was convicted several times of incitement to racial hatred, has moved towards the conservative mainstream and is now fit to govern.
With 89 lawmakers, the RN is the second-biggest party in the parliament.
A close ally of Le Pen, Jordan Bardella, is expected to take over as the party leader on Saturday - even if Le Pen still calls the shots.
"The National Rally has shown its true face today," the Nupes left-wing alliance said in a statement. "This racist slur is characteristic of the far-right: stigmatise according to the colour of your skin, divide the French people."
