Unlike similar missions to Mars, which focus on finding signs of ancient life long since extinguished, scientists hope Jupiter's icy moons will still be home to living organisms, even if only tiny or single-celled.
Such habitability requires a power source. Lacking energy from the Sun, the moons could instead take advantage of the gravity that Jupiter exerts on its satellites.
The force creates a process called tidal heating, which warms the interior of the moons and keeps their water liquid.
Ganymede's "gigantic" liquid ocean is trapped between two thick layers of ice dozens of kilometres beneath the surface, said Carole Larigauderie, JUICE project head at French space agency CNES.
"On Earth, we still find life forms at the bottom of the abyss," she added.
Tiny microbes such as bacteria and archaea have been found to be able to survive on Earth without sunlight, raising hopes that life elsewhere will be able to do the same.
As well as water and energy, life needs nutrients.
"The big question is therefore whether Ganymede's ocean contains" the necessary chemical elements, Coustenis said.
The ocean would need to be able to absorb the nutrients from anything that fell on the moon's surface, for example, which would eventually dissolve into the water, she added.
Not alone
JUICE's array of instruments will probe Ganymede's ocean to determine its depth, distance from the surface and -- hopefully -- its composition.
The ESA's 1.6 billion euro ($1.7 billion) probe will spend eight months orbiting Ganymede, getting as close as 200 kilometres (125 miles) from the moon, all while sheltered from radiation.
It will not be the only spacecraft lurking around Jupiter.
NASA's Europa Clipper mission is scheduled to launch in October next year. It will take a quicker path to Jupiter, arriving at Europa in 2030.
If one -- or more -- of Jupiter's moons ticks all the boxes to host life, the "logical next step" would be to send a mission to land on the surface, said Cyril Cavel, JUICE project manager at manufacturer Airbus.
Although there are no plans for such a mission, which could definitively prove the existence of life outside of Earth, "that's part of the dream," he said.