The Colombian performer, 46, stole the show as she performed her signature hip swings and pops, singing a bilingual medley including "She Wolf," "Te Felicito," "Objection" "Whenever, Wherever" and "Hips Don't Lie.
Rapper Nicki Minaj hosted the event for the second consecutive year, also debuting live her newest single, "Last Time I Saw You," which is slated for release on her album "Pink Friday 2" in November.
She won the night's award for Best Hip Hop, for "Super Freaky Girl."
