At a rally in the capital Brasilia on Tuesday, more than 6,000 indigenous Brazilians urged the country's Supreme Court not to touch their ancestral areas and to allow the establishment of new protected areas.
Demonstrators gathered outside the court building used 380 lamps to spell out the words "Brazil, indigenous land," according to a statement from the indigenous umbrella organization Apib.
On Wednesday, the Supreme Court is expected to rule on the indigenous people's claim to their ancestral land.
The court decision is about a legal argument known as the Marco Temporal which indigenous advocates fear could legalize the theft of indigenous land.
Business interests that want to exploit indigenous land for mining and industrial agriculture use the Marco Temporal to argue that indigenous peoples must prove they occupied the lands in question when Brazil's constitution was adopted in 1988.
Wednesday's ruling will guide the federal government and future courts in settling indigenous land issues and addressing indigenous rights.
Indigenous representatives have been staging protests in Brasilia since Sunday ahead of the ruling.
They have also demonstrated in front of the National Congress, carrying posters with slogans like "Bolsonaro out."
Right-wing President Jair Bolsonaro is a proponent of the economic exploitation of the Amazon region and announced in the 2018 election campaign that he would no longer designate an inch of it as a protected area.
